Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. • Specific for dsDNA in the presence of RNA. According to the obtained results, the assay presents a linear correlation in a wide range of 0. 微孔板读数仪. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Prepared DNA concentration of the samples was 250 ng/ mL. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The assay is linear over three orders of magnitude and has little sequence. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. The range of linearity for DNA concentration measurements. PicoGreen dsDNA quantitation reagents are. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 3 Q33120 39. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Catalog Number. Highly selective for dsDNA over ssDNA or RNA. The libraries were sequenced using the Illumina HiSeq 2500. 99. Coleen - 06 Oct. Buy from Supplier. high-range. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. high-range. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. 0 ml 200x in DMSO b. PicoGreen dsDNA quantitation reagents are. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. • Suitable for use in 96- and 384-well plate formats. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. • Specific for dsDNA in the presence of RNA. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Natural cures with herbs are. • Specific for dsDNA in the presence of RNA. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Blog May 11, 2021. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Picogreen assays are compatible with most microplate readers and fluorometers. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Rapidly detect as little as 0. Plasma levels. Quantitation Range. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). For a standard. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Picogreen assays are compatible with most microplate readers and fluorometers. Description. Bioz Stars score: 86/100, based on 1 PubMed citations. Unit Size. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. dsDNA만을 선택적으로 정량. Fluorescence was measured at. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Both fluorescent assays were performed with a Qubit fluorometer. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 10 x 100 µL. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. Figure 2. 2023. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. DNA standard curve. 5 Q32851 15. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 実験してみた – PicoGreenをプレートリーダーで使用してみた. 0. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. 适用于（设备）. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 125 to 100 ng/mL. Showing 4 of 4. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Suitable for use in 96- and 384-well plate formats. 0. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Barcoded amplicons were pooled in. 2. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. Add 5uL of PicoGreen reagent for every 1mL of buffer. '. • Specific for dsDNA in the presence of RNA. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Picogreen assays are compatible with most microplate readers and fluorometers. 1 mL kit. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. Unit Size. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. 3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quantify nucleic acids and proteins using four user-selectable fluorescent. This was possible since, due to the low height of the light ray of only 8. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. assay. P11495. Quant-iT PicoGreen dsDNA Assay Kit a. Quant-iT PicoGreen dsDNA Reagent. 1 mL kit. g. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. Mix PicoGreen and DNA by pippetting up and down 5-10 times. Catalog Number. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. Begin typing to search, use arrow keys to navigate, Enter to select. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. (The same tips can be used. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. Description. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Table 2 summarizes results of the. Bioz Stars score: 86/100, based on 1 PubMed citations. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). 8 Measurement of growth factor content in. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. Breadcrumb. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. PicoGreen dsDNA quantitation reagents are. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Reagent Search & Data Services Menu Reagent Search. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Quant It. Picogreen Reagent b. Figure 2. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. 99 to $1999. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. 99 to $1999. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. This protocol is a simplified and condensed version of. This assay is designed for use with fluorescence microplate readers. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. PicoGreen dsDNA quantitation reagents are. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Quantitation Range. low-range. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. 10 ng/mkl – 10000 ng/ml. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. PicoGreen®試薬アッセイ. 50 pg to 2 µg. 2 to 100 ng) Assay Kit were used with a Qubit 3. The tubes were. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). This protocol is a simplified and condensed version of. 2023. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Bioz Stars score: 86/100, based on 1 PubMed citations. Rapidly detect as little as 0. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. no. • Suitable for use in 96- and 384-well plate formats. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. • Specific for dsDNA in the presence of RNA. 0 fluorometer and Quant-iT PicoGreen dsDNA. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. DNA. Catalog Number. This kit contains. This sensitivity exceeds that achieved with the Hoechst 33258. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. Bioz Stars score: 86/100, based on 1 PubMed citations. 5 ng/mkl – 100 mg/ml. 7. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. Rapidly detect as little as 0. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . 0 ml 200x in DMSO b. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. It must be completely liquified before use by allowing it to come to room temperature. Abstract. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Fluorometric dsDNA Assay. 04 corresponds to 2 μ g/mL dsDNA solution. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). (D–G) TLR stimulation was measured using a HEK293-TLR reporter. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. In case dsDNA standards are available only the quantification reagent can be used. For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2. AccuBlue® RNA Quantitation Kit Details. Product Type. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Rapidly detect as little as 0. The PicoGreen is sticking to the sides of the cuvette or assay plate. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. Figure 8. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays*Selection Guide for the Qubit and Quant-iT Assay Kits—Table 8. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. The accuracy index is defined in Table 2. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. 7 Q33231 6 1,000 41. DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. 2009;49(4):997–1002. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Quant-iT PicoGreen dsDNA Assay Kit. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. Thermo Fisher quant it picogreen dsdna assay kit. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. PicoGreen Assay: CHO DNA Concentrate (10 µg/mL), 120 µL D553X Quant-iT PicoGreen dsDNA reagent D003 Solution, 1 x 1. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Quant-iT PicoGreen dsDNA Assay Kit. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen assay. Picogreen assays are compatible with most microplate readers and fluorometers. 21 DiB) [22]. 激发/发射. 製品番号（カタログ番号）： P7581. All LNP samples were diluted 300-fold in TE buffer and placed on a Corning ® 96 Well Solid Polystyrene Microplate (Sigma-Aldrich, St. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Buy from Supplier. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. Rapidly detect as little as 0. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. Picogreen assays are compatible with most microplate readers and fluorometers. • Suitable for use in 96- and 384-well plate formats. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Quantitation using Quant-iT Assay Kits. Dilute from 20xTE stock (supplied in the PicoGreen kit). Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration. Quantitation Range. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). • Specific for dsDNA in the presence of RNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Rapidly detect as little as 0. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). Average 86 stars, based on 1. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Rapidly detect as little as 0. 99 to $1999. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. Bioz Stars score: 86/100, based on 1 PubMed citations. Product Type. Pico488 allows to quantitate as little as low pg/mL of dsDNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 2–100 ng, the fluorescence signal is linear with DNA. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 50 pg to 2 µg. Table 3. 9. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. 1. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). 2. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. 10. Picogreen assays are compatible with most microplate readers and fluorometers. 1X dsDNA assay (%) 100 17. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. 99. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Description. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. 10pg/µl vs. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 2023-11. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Catalog Number. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). 995 mL TE for the . 2–100 ng: 2-1,000 ng: 0. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Louis, MI, USA). o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. The assay is linear over three orders of magnitude and has little sequence dependence. • Specific for dsDNA in the presence of RNA. The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. • Specific for dsDNA in the presence of RNA. PicoGreen Solution must be diluted fresh before each assay run. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free.